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#P2Solo @nanopore second trial of rapid PCR cDNA sequencing, running 18 samples on a PromethION P2 Solo flow cell.

[Our first trial was on a MinION flow cell]

I'm hoping to get over 25M reads from this run, which should be easily achievable given that it should get to 2M reads within the first hour, and it'll be running for 72 hours.

My current estimate is for about 70M reads by the end of the run.

Pore activity plot for the sequencing run, demonstrating about 60-70% pore occupancy (fairly typical for this version of cDNA sequencing).
Channel states panel for the sequencing run, showing a speckled image that is mostly green and cream (sequencing / available), with a sprinkling of purple (active feedback). There is a local grouping of light blue (zero pore) states the left-hand side of the left-most channel region, suggesting a physical issue with the flow cell (e.g. a small bubble).
#p2solo @nanopore group
in reply to Frank Aylward

David Eccles ๐ŸŒป๐Ÿ‡ต๐Ÿ‡ธ6x๐Ÿฉน๐Ÿ›ก๏ธ
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David Eccles ๐ŸŒป๐Ÿ‡ต๐Ÿ‡ธ6x๐Ÿฉน๐Ÿ›ก๏ธ
• •
@foaylward not planning to reload at the moment. We've got one barcode that's producing about 100x the reads of the lowest-yield sample, so need to rerun without that sample anyway. If things look like they're tailing off, we might think about reloading for the next run.
@Frank Aylward
in reply to Frank Aylward

David Eccles ๐ŸŒป๐Ÿ‡ต๐Ÿ‡ธ6x๐Ÿฉน๐Ÿ›ก๏ธ
mastodon - Link to source
David Eccles ๐ŸŒป๐Ÿ‡ต๐Ÿ‡ธ6x๐Ÿฉน๐Ÿ›ก๏ธ
• •

@foaylward long reads gel up the pores pretty quickly, which *is* a problem that we're having with this run, but it's not worth our time / money to re-use this flow cell for the same samples.

I'll probably wash it out and re-use it for lower-volume samples that I would have previously used a Flongle flow cell for.

@Frank Aylward

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