A #preprint detective story
"Our two laboratories encountered independently sudden-onset, major impediments to such research. Spore suspensions and vegetative cells no longer plated effectively on minimal media. By systematically analyzing multiple different media components from multiple different suppliers, we identified the source of the problem"
https://www.biorxiv.org/content/10.1101/2024.06.06.597796v1
"Our two laboratories encountered independently sudden-onset, major impediments to such research. Spore suspensions and vegetative cells no longer plated effectively on minimal media. By systematically analyzing multiple different media components from multiple different suppliers, we identified the source of the problem"
https://www.biorxiv.org/content/10.1101/2024.06.06.597796v1
Laboratory horror stories: Poison in the agars
The fission yeast Schizosaccharomyces pombe is a single-celled eukaryote that can be cultured as a haploid or as a diploid.bioRxiv