Tools
"Here, we engineered tobacco rattle virus to carry the compact RNA-guided TnpB enzyme ISYmu1 and its guide RNA. This innovation allowed transgene-free editing of #Arabidopsis thaliana in a single step, with edits inherited in the subsequent generation. "
#Preprint
biorxiv.org/content/10.1101/20…
"Here, we engineered tobacco rattle virus to carry the compact RNA-guided TnpB enzyme ISYmu1 and its guide RNA. This innovation allowed transgene-free editing of #Arabidopsis thaliana in a single step, with edits inherited in the subsequent generation. "
#Preprint
biorxiv.org/content/10.1101/20…
Viral delivery of an RNA-guided genome editor for transgene-free plant germline editing
Genome editing is transforming plant biology by enabling precise DNA modifications. However, delivery of editing systems into plants remains challenging, often requiring slow, genotype-specific methods such as tissue culture or transformation.bioRxiv