Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes

nature.com/articles/s41564-025…

#viruses #SingleCell #marine #ocean

Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes - Nature Microbiology

Environmental micro-compartment genomics provides efficient and high-throughput single-particle DNA sequencing that captures overlooked members of microbial communities.

^Nature

Who are giant viruses infecting in the ocean?

In a collaborative paper with the Vardi lab, we were able to link many giant virus lineages to protist hosts using #SingleCell #RNASeq. One #protist host (leucocryptos) made up less than 1% of the community, but we could still identify a cryptic viral lineage infecting it, leading to population collapse.

nature.com/articles/s41564-024…

#marine #viruses #microbiology

Single-cell RNA-seq of the rare virosphere reveals the native hosts of giant viruses in the marine environment - Nature Microbiology

Active infections of giant viruses in their marine protists hosts are tracked at single-cell resolution, showing that, despite being rare, these viruses still impact microbial population dynamics.

^Nature

Significance analysis for clustering with single-cell RNA-sequencing data

nature.com/articles/s41592-023…

#sc #singlecell #rnaseq

Significance analysis for clustering with single-cell RNA-sequencing data - Nature Methods

This study presents a significance analysis framework for evaluating single-cell clusters. Application of the method detects cases of over-clustering in reported single-cell RNA-sequencing analysis results.

^Nature