Who are giant viruses infecting in the ocean?

In a collaborative paper with the Vardi lab, we were able to link many giant virus lineages to protist hosts using #SingleCell #RNASeq. One #protist host (leucocryptos) made up less than 1% of the community, but we could still identify a cryptic viral lineage infecting it, leading to population collapse.

https://www.nature.com/articles/s41564-024-01669-y

#marine #viruses #microbiology

Single-cell RNA-seq of the rare virosphere reveals the native hosts of giant viruses in the marine environment - Nature Microbiology

Active infections of giant viruses in their marine protists hosts are tracked at single-cell resolution, showing that, despite being rare, these viruses still impact microbial population dynamics.

^Nature

Significance analysis for clustering with single-cell RNA-sequencing data

https://www.nature.com/articles/s41592-023-01933-9

#sc #singlecell #rnaseq

Significance analysis for clustering with single-cell RNA-sequencing data - Nature Methods

This study presents a significance analysis framework for evaluating single-cell clusters. Application of the method detects cases of over-clustering in reported single-cell RNA-sequencing analysis results.

^Nature