We have a new preprint online! A great collab with the Tresch lab in Cologne. We found that mRNA 🧬 export out of the nucleus is surprisingly slow and and a rate limiting step for mRNA metabolism. Take a look: https://www.biorxiv.org/content/10.1101/2023.05.04.539375v1
#ScienceMastodon #RNA #mRNA #preprint #mpinat
#ScienceMastodon #RNA #mRNA #preprint #mpinat
Nuclear export is a limiting factor in eukaryotic mRNA metabolism
The eukaryotic mRNA life cycle includes transcription, nuclear mRNA export and degradation. To quantify all these processes simultaneously, we perform thiol-linked alkylation after metabolic labeling of RNA with 4-thiouridine (4sU), followed by seque…bioRxiv