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Items tagged with: scrnaseq
Single cell RNA-sequencing (#scRNAseq) is an essential method to learn about cells in health and disease. Here we have studied "multiplets", an important source of error of scRNAseq. We find that multiplets are astonishingly frequent and hard to eliminate.
doi.org/10.1101/2025.06.09.658…
doi.org/10.1101/2025.06.09.658…
My first post on Mastodon after a whole lot of lurking and boosting, so I'm treating this as a mini #introduction for myself
I'm an #immunology PhD candidate at #ColumbiaUniversity medical center, and we just published a study of #TissueSpecifity of human #Tcell identity and clonal expansion in barrier sites, using #CyTOF, #scRNAseq, and #TCR #sequencing!
nature.com/articles/s41590-022…
Tissue adaptation and clonal segregation of human memory T cells in barrier sites - Nature Immunology
Farber and colleagues examine the phenotypic, transcriptomic, clonal, and functional differences between tissue-resident T cells in various barrier tissue sites relative to T cells in lymphoid organs and circulation in humans.Nature