#Bacteriophages encode anti-CRISPR proteins that inactivate bacterial #CRISPR-Cas systems, allowing successful invasion, replication & integration. This study shows that #antiCRISPR protein AcrIIA1 induces degradation of #Cas9 in human cells #PLOSBiology plos.io/3uUGAos

A diverse set of species, from #snails to #algae to #amoebas, make programmable DNA-cutting enzymes called #Fanzors — and a new study from scientists at MIT’s McGovern Institute for Brain Research has identified thousands of them.
#CRISPR #DNA #RNA #Microbiology #sflorg
sflorg.com/2023/10/mcb10142302…

#Rotifers have been studied for >100 years re #microevolution, #EcologicalDynamics & #ecotoxicology. This study presents a #CRISPR-based method for KO mutation of 1 or 2 alleles, and KI mutations, in rotifers with high efficiency @MBLscience #PLOSBiology plos.io/3Kb8CAC

Restriction endonuclease cleavage of phage DNA enables resuscitation from Cas13-induced bacterial dormancy
nature.com/articles/s41564-022…
#phage #bacteria #crispr

Many in vitro #CRISPR knock-in approaches use small molecule drugs to improve the efficiency of homology-directed repair, but these drugs may or may not be safe in vivo. In this paper from #NatureBiotechnology, the authors find that an FDA-approved therapy, fludarabine, can be used for a similar purpose. Still a long ways to go before any genetic knock-ins are routinely done in vivo, but making progress toward doing so safely: nature.com/articles/s41587-022… #science #medicine #GeneEditing #AAV