Multiple checkpoints ensure ribosomes have the correct end

The 3’ end of 18S ribosomal RNA is formed by the endoribonuclease Nob1, but how cells ensure the accuracy of the 3’ end has been a mystery.

An ancient bacterial zinc acquisition system identified from a cyanobacterial exoproteome

Bacteria have developed fine-tuned responses to cope with limiting amounts of the essential mineral zinc.

How do bacterial endosymbionts work with so few genes?

The move from a free-living environment to a long-term residence inside a host eukaryotic cell has profound effects on bacterial function.

Cas9 degradation in human cells using phage anti-CRISPR proteins

Bacteriophages encode anti-CRISPR proteins that inactivate CRISPR-Cas bacterial immune systems, allowing successful invasion, replication, and prophage integration.

Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages

In this work, authors use an arrayed genome-wide CRISPR interference assay to map gene essentiality landscape in the canonical coliphages λ and P1.

Resurrection of 2′-5′-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication

The human OAS1 protein restricts SARS-CoV-2 replication, but the horseshoe bat OAS1 has lost this ability.

How many species are there on Earth? Progress and problems

How many species are there on Earth? Projections have ranged from the millions to the trillions. This Perspective looks at how a 2011 PLOS Biology study transformed this field and what is still to be done to find a definitive answer.

Components of iron–Sulfur cluster assembly machineries are robust phylogenetic markers to trace the origin of mitochondria and plastids

The symbioses that gave rise to primary organelles are key events in the origin of Eukaryotes. This study uses a dataset of markers belonging to two conserved machineries responsible for [Fe-S] cluster biogenesis to support an early emergence of mito…

Multiple light signaling pathways control solar tracking in sunflowers

Sunflowers are famous for their ability to track the sun throughout the day and then reorient at night to face east the following morning.

Transient eco-evolutionary dynamics early in a phage epidemic have strong and lasting impact on the long-term evolution of bacterial defences

Organisms use a range of defence systems to protect against their parasites. Using a bacteria-phage model system, we investigate what drives the transient evolution of different systems, which has important implications for host ecology and pathogeni…

The human fungal pathogen Aspergillus fumigatus can produce the highest known number of meiotic crossovers

Aspergillus fumigatus is a saprotrophic fungus that can cause serious life-threatening invasive infections in immunocompromised individuals. By constructing a recombination map, this study shows that A.

Supporting nonlinear careers to diversify science

Those who follow non-linear career trajectories often face disadvantages in academia. This Perspective looks at why individuals might choose non-linear careers and how these benefit diversity in science.

Relationship between journal impact factor and the thoroughness and helpfulness of peer reviews

An analysis of the content of 10,000 peer review reports reveals that reports submitted to journals with higher impact factors pay more attention to the materials and methods of a study but less attention to presentation and reporting, whereas journa…

Functional unknomics: Systematic screening of conserved genes of unknown function

The human genome contains many genes whose function remains mysterious, despite decades of research; this study presents a publicly available “Unknome database” and reveals that genetic screens that focus on this "unknome" can help shed light on fund…

Highly efficient CRISPR-mediated gene editing in a rotifer

Rotifers have been studied in the laboratory and field for over 100 years in investigations of microevolution, ecological dynamics and ecotoxicology.

Plant virus movement proteins originated from jelly-roll capsid proteins

Movement proteins, a signature of plant RNA and DNA viruses, enable cell-to-cell movement of the virus via plasmodesmata channels.

Cheating leads to the evolution of multipartite viruses

In multipartite viruses, the genome is split into multiple segments, each of which is transmitted via a separate capsid.

Save the planet with green industries using algae

We can use photosynthesis to capture carbon and make industries greener. Algae-driven carbon capture and manufacturing offer the potential for reducing CO2 emissions while also producing commodities such as bioplastics.

A male-killing Wolbachia endosymbiont is concealed by another endosymbiont and a nuclear suppressor

Wolbachia bacteria live in the cells of many insects and have a variety of reproductive effects on their host.

Behind the paper: human collaboration to study virus interactions - PLOS Biologue

In this ‘behind the paper’ post, Anna Sims discusses her experience working in a virology lab during the height of the COVID-19 pandemic while not working in SARS-COV-2, and the importance of collaboration and discussion with peers in science.

Four principles to establish a universal virus taxonomy

Transforming an existing phenotypic classification of viruses into one based on evolutionary relationships that can accommodate the vast number of viruses characterized in metagenomics and environmental studies is an ongoing challenge.

The dawn of relaxed phylogenetics

In 2006, a landmark study on relaxed phylogenetics was published in PLOS Biology. This Perspective discusses how relaxed phylogenetics has influenced the field of evolutionary biology by enabling researchers to investigate evolution’s tempo.

Viral infection in the ocean—A journey across scales

Viruses are the most abundant biological entity in the ocean. This Essay journeys across several orders of magnitude of biological organization, time, and space, to investigate interactions between hosts and viruses in the ocean and their relevance f…

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